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JOJ p. Ill p. Sllr fe pflltc'tlll ri'A tlois-. H ISlmre cl son. Cahie" de b Maison de hanheuc ct de l'architL'Ctllrc : La Vie du rail cl des trJnspom. Cr':it'Jduw't: 5. P J Cltl. J:lotfl1J l:t J ll. P tru o n rw:'I JII. LN IVS Dan Dylan. Published on Apr 9, I n d ividual But even hundreds of cells cells learn to c ommunicate may not be enough to allow with c o mputer a l g orithms someone to control two mem ore effectively over t i m e chanical limbs at once — the by changing their firing patdevice that scientists hope to terns, as revealed in a study showcase at the World Cup.
That's why his team. Made of a flexible plastic that conducts electricity, the electrode can monitor nearly 2, brain cells in a mouse. Even as scientists explore t he brain's ability t o m a k e prosthetics m o ve , t h e y 're thinking ahead to the next step. Some want the brain to receive messages from prosthetics that feel. His team's artificial hand, intended to be worn by amputees, connects not to the brain itself but to peripheral nerves near the end of a severed arm.
Flexible electrodes threaded into those nerves provide a two-way street for information. Brain signals traveling outward can close the false hand's five fingers, pinch the index finger and thumb together and wiggle the little finger. Sensors on the prosthetic's palm send information back to the brain, providing a sense of pressure.
In Nicolelis' lab, monkeys can feel virtual objects displayed on a computer screen when areas of the brain associated with the sense of touch are stimulated.
The blueprints for nextsummer's soccer exoskeleton include sensors that will provide an artificial skin for its human wearer. With the world watching, Nicolelis hopes not only that his bionic teenager will be able to feel the ball but also that disabled people everywhere will feel a sense of hope.
Because Moran's electrodes are positioned farther from the Extending the lifetime action, they pick up less detail; Upping the number of neu- it's like someone trying to lisrons might also help extend ten to a conversation through the i m p r actically m e a ger a door. But with amplifiers lifetimes of neuroprosthetics.
In the first human test, die ormove out of range. Acreported Feb. Other technologies might minimize that deterioration. Louis, Daniel Moran places Painting in Central Oregon for over 18 years electrodes on the surface of the brain instead of implanting them within it. OSU-Cascades takes science out of the lab and into your local pub! No scientihc background required — just bring your curiosity, sense of humor and appetite for food, drinks and knowledge!
Learn how these invaders arrive in ways vve would never anticipate — through innocent classroom projects, gardening, and on floating tsunami debris — and what you can do to prevent and minimize their impact. Chan's research guided the creation of the award-winning statewide "Silent lnvasion" program. Due to space, attendance limited to Doors open at P. Presentation starts at P.
There will be a public hearing at 10 a. June 5 at the commission meeting, where residents can address the proposed SDC fee schedule. The resolution would also add a s eparate designation for homes built in destination resorts,add a reimbursement fee, establish that SDCs are assigned to land rather than.
A dramatic backlash Their anxieties were intensified by George W. Bush's aggressive advocacy of a "freedom agenda," which called for democratic transformation in the Arab world, and President Barack Obama's support for the "Arab Spring" revolts that toppled o r c h a llenged leaders in the Middle East and North Africa. The backlash ha s b e en dramatic. About 50 countries have adopted laws to limit for-.
B aney suggested at t h e Wednesday w o r k se s sion that homebuilderswere overcharged by the Road Department between and when paying t h e c o unty's transportation system development charge due to overprojections o n po p u l ation growth.
The amount was determined using a mathematical equation based on projected population growth. When the housing bubble burst in and growth slowed in the area, the projected numbers, and, rights are gone.
But the effort ignited a struggle between Washington and postrevolutionary governments in Cairo. They also took computers and recordsfrom localoffices of the National Democratic Institute and the International Republican Institute, Washingtonbased nonprofit groups that seek to support democratic institutions worldwide. The crackdown apparently drew broad public support. Continued from A1 Once complete, it will allow trail users on the east side of the river to link to the trail system on the west side.
First Street rapids T he new f o o tbridge i s said. It i s a of seven feet clearance in "free-spanning" bri d g e, very high water," he said. This trail users on the west side , Pioneer creates fewer obstacles for near Revere Avenue will be portlandAve. It also simplified the the existing trail on the west permitting process for build- side to cross Portland Aving the bridge, Erwert said.
At Portland Avenue, The state permits crews to southbound trail users will work in the river only a few cross the river once more. Analysts say it's easy to understand why many governments, and their citizens, are suspicious of the pro-demo-. We are Criminal charges are still intervening directly in their pending i n C a i r o a g a inst political affairs.
Valid Sun-Thurs, Now - May 23, Food donated to Lincoln County Food Share. The senior Obama administration official said Egypt has allowed some civil society groups to continue operating, including those that work on f ai r elections and educatingyoung people about democracy. In Bolivia, state news agencies blamed USAID for "alleged political i n t erference w ith p easant u n i ons a n d s ocial o r ganizations.
About 3 0 o t h e r 85 percent of those surveyed countriesare considering restrictions, according to the International Center for Not-forProfit Law, a research group in Washington. Russian President V l adimir Putin clearly has a different view. But U. Russia also has kicked out UNICEF, the United Nations children's fund, and investigated a bird conservation society to see if it poses a threat to public order.
If you want a multi-point vehicle check-up, tire rotation with brake inspection, air andcabin filter replacement, wiper blade replacement, headlampand light bulb replacement and abattery test Nobodyknowsyour vehicle better! Richard Krasuski, a carcans: Owning a dog may prodiologist at the Cleveland Clintect you from heart disease.
The cular risk. The heart association pubThe new report reviewed People who own dogs cer- lishes about three scientific dozens ofstudies, and overall tainly have more reason to go statements a month, typically it seemed clear that pet ownoutside and take walks, and on more technical matters, ers, especially those with dogs studies show that most own- but the group was prompted the focus of most of the studersform such close bonds with to take a stance on the pet is- ies , were in better health than their pets that being in their sue by the growing number people without pets.
Some research showed that people who had pets of any kind were also more likely to survive heart attacks. Riley, in New York. On Thursday, the American Heart Association And the research also strong- published a scientific statement that owning a dog may protect ly suggested that among dog from heart disease. Levine said that he and his colleagues were not recommending that people adopt pets for any reason other than to give them a good home.
States that also handles Visa T he hackers — wh o a r e and M a s terCard p r e paid not named in the indictment debit cards. The company's — proceeded to raise the with- name was not revealed in the drawal limits on prepaid Mas- indictment.
Starting at 3 p. While the indictment suggestsa far-reaching operation, there are no details about the peopleresponsibleforconducting the computer hacking or who might be leading the global operation. Law enforcement agencies in more than a dozen countries, including J apan, Canada, Germany and Romania, have been involved in the investigation, prosecutors said.
T he authorities said t h e leader of the New York cashing crew was Alberto Lajud-Pena, 23, who also went by the name Prime. His body was found in the Dominican Republic on April 27and prosecutors said they believe he was killed. Seven other people were charged with conspiracy to commit "access device fraud" and money laundering. The prosecutors said they were all U. The indictment says the defendants "invested the criminal proceeds in portable luxury good, such as expensive watches and cars.
Tribes Continued from A1 On the same day the law took effect, two Navy veterans became the first couple — and so far, the only — to take advantage of it: Tim LaCroix, 53, a tribalmember, married Gene Barfield, 60, his partner of 30 years. Opponents are criticizing the new law in the current tribal elections. He ran for the tribal council but lost, though he hopes there's enough turnover to scrap the law after the June general election.
John Keshick, a member of the council who voted against gay marriage, said the tribe should accept the outcome. In one of the first votes, the Navajo Nation Council in banned same-sex marriages and unions b etween close blood relatives. Three years later, the Coquille Indian Tribe in Oregon became the first in the nation to approve a gay marriage law. The federal law allows states. Purser, a member of the Suquamish Tribe, got her tribal council to vote in favor of gay marriage.
Whitener said many tribes are hesitant to pass laws because of the hard legal issues involved: For example, does a tribe have jurisdiction over the divorce of a tribal member who married a non-tribal member on reservation land'? Scholars note that before their introduction to Christianity, many tribes accepted their gay, lesbian, bisexual and transgender members as"two spirits," even giving them added respect because they were thought to have special powers. Marie Tribe of Chippewa Indians of Michigan.
He said tribal members have "a much more fluid spirituality" and tend to be more tolerant. The May 21 ballot carries contests extremely.
The Bulletin will publish a daily calendar of election-related events, including candidate. Are you planning an event? Box , Bend OR Jason Conger is worried that Oregon has put out the welcome mat for sex offenders. The Republican from Bend teamed up with state Rep. Andy Olson, R-Albany, a former Oregon State Police trooper, to create legislation that would require anyone convicted of sex crimes outside ofOregon to register as an offender once he or she moves here.
State law already requires offenders convicted of sex offenses within Oregon to register. Conger said he was surprised to learn that sex. HouseBill What it does:Requires anyone convicted of a crime that would require him or her to register as asexoffender in another state to do the same in Oregon. Applies to both state and federal crimes;.
The way the law is currently crafted, he said, almost offers an incentive for anyone convicted of such crimes to move to Oregon. If passed, the law only applies to crimes committed afteritspassage. Aaron Knott, legislative director with the attorney general's office, last month told lawmakers the law would deny sex offenders a "safe haven" in Oregon. It would also require people convicted of federal sex crimesto register as sex offenders at the state level, which they currently do not have to do, according to Michael Slauson, an attorney with the state Department of Justice.
Other than through anecdotal evidence, Conger said, he does not know how often sex offendersactually move to the state. But, he said, he believes other states are mov-. Olson, the former police officer, said he was also unaware until recently that sex offenders did not have to register in the state if they had committed the crime elsewhere. Fundraising events do not qualify, nor do strictly.
A map for the mile ride looping through Madras, Metolius and Culver is already available online, and by the end of June ' Mapof there should route,B5 be signs up,.
Ballots Ballots must be received by county elections officials no later than 8 p. Postmarks do not count. The Madras- Jefferson County Chamber of Comm erce supported the plan for the scenic bikeway, the proposal for which came from Stan Nowakowski, 57, and Maura Schwartz, The Madras couple ride at least a section of the route once a week.
They look forward to having company now that it is a scenic bikeway. Well shot! Submit your best work at www.
Submission requirements: Include as much detail as possible —when and where you took it, and any special technique used — as well as your name, hometown and phonenumber. Photos m ust be high resolution at least 6 inches wide and dpi and cannot bealtered. Redmond's first school bell has been on display at Vern Patrick Elementary for nearly 20 years. This wasn't the first time. Redmond's Historic Landmarks Commission has been looking for ways to preserve asmall schoolhouse, currently locatedin the Dry Canyon, and decided that awareness of this effort would be a good way to celebrate May's National Historic Preservation Month.
And in keeping with the education theme, members decided to track down a historic school bell thought to be somewhere in the community. They hoped to install it in a display at Redmond Library, a building that had served as an elementaryschoolformore than 65 years. Commission members Jane Schroeder and Trish Pinkerton started working.
One lead ended at the Redmond Historical Society, which had a bell in its storage area but it was from a train, not a school. Schroeder called former Redmond librarian Ardyce Swift and Pinkerton called the school district, and that's when everything fell into place: The bell wasn't lost at all, just forgotten. Bell origins Before , school classes in Redmond were held in v arious commercial and residential locations. But in , the classes got their own wooden building, sited roughly where the library now stands.
The school'ssole teacher, Alwilda Wilson, started a fundraising drive for a bell that could be heard all through — and outside of — the fledgling community. According to author Elizabeth Ward's. But the bell was eventually purchased and used at the new school.
The school grew in size over the years, including a tower installed in The bell hung there until , when a new brick schoolhouse, later named Jessie Hill Elementary, was constructed next door. T he bell was stored away i n t h e basement. In when her daughter was a student at the same school, the bell, which had lost its dedication plaque, was "discovered" by a custodian.
Construction crews are set to begin work on the rebuilding of Reed Market Road next week. The City of Bend hosted an open house Thursday night to brief local residents on the project. Crews will begin work on the eastern portion of the project between Newberry Drive and 27th Street starting next Wednesday, with work on the western portion not due to begin until September. The entire project is not scheduled to be completed until late summer Nick Arnis, transportation engineering manager for the.
The handful of local residents who trickled through Thursday's open house were largely positive in their conversations with city staff and contractors hired to design and build the project.
Notably, Reed Market Road will be built with concrete rather than the asphalt used on nearly every other road in Bend. This phase is divided into three stages:QNewberry. Drive to thewest side of ShadowoodDrive MayAug. Continued from B1 "No one really paid it much attention; it didn't seem that important then," said Hassler.
Her stepmother was Wilson's sister. But when the bell was found after 20 years ofneglect, it was refurbished with donations by the Wilson family and mounted on a frame inside the Jessie Hill courtyard. Any new information, such as the dismissal of charges or acquittal, must be verifiable. For more information, call Aside trip The bell remained in t he courtyard until the late s, when a group of Redmond High School athletic boosters spotted it and thought it would.
The schools superintendent at the time, Howard Kearns, gave permission for the bell to be removed and presented to the students at RHS. The bell was sandblasted, p ainted a b r i ght g old a n d mounted in a maroon frame constructed by students.
As far as Schiel remembers, the. History lovers and members of the Wilson family heard about the bell's new home and protested. Eventually, the school district reversed its decision and had the bell returned to Jessie Hill. The sole teacher at Redmond's first permanent school, Alwilda Wilson, started a fundraising drive for a bell that could be heard throughout the community. Lost in plain sight When the Redmond School District decided to close Jessie Hill in , plans were made to move the bell to the new grade school, Vern Patrick.
The school's design was altered to add a tower for the bell, but no signage or information was installed to inform the public about the history of the bell, so except for students and staff, the bell was largely forgotten. That's where Swift came in. She w a s h e a d l i b r arian when the transition from school to library was made, so she pointed Schroeder to-. I think building. Jeff Merkley, D-Ore. Hawthorne Ave. RottWydett, D-Ore.
Salem, OR Phone: Email: oregon. Oregon St. Third St. McPherson ci. US Email: broppe cityofprineville. Wall St. CascadeAvenue, P. Box , Sixth St. Theft — A theft was reported at p. May 1, in the block of Northwest Wall Street. May 1, in the block of Northeast Second Street. Criminal mischief — An act of criminal mischief was reported at p.
May 2, in the block of Northeast Franklin Avenue. May 3, in the block of Northeast First Street. Theft — A theft was reported and an arrest made at p. May 3, in the block of Pinebrook Boulevard. Criminal mischief — An act of criminal mischief was reported at a. May 5, in the block of Rock Bluff Lane. Criminal mischief — An act of criminal mischief was reported and an arrest made at a.
May 6, in the block of Northeast Third Street. Unlawful entry — A vehicle was reported entered at11 a. May 6,in the block of Lysander Place. May 6,inthe block of North U. Highway Unlawful entry — A vehicle was reported entered at p.
May 7,in the block of Clausen Drive. Unauthorizeduse — A vehicle was reported stolen and an. May 7, in the block of Green Lakes Loop. May 7, in the block of Northeast Division Street. May 7, in the block of Columbine Lane. May 7,in the block of Northeast 18th Street.
Burglary — A burglary was reported at p. May7, in the block of Pikes Court. Theft — A theft was reported atp. May7, inthe block of Northwest Foxwood Place. May 7, in the block of Northeast 11th Place.
May 8, in the area of North Main Street. May 8, in the area of Northeast Third Street. Vehicle crash — An accident was reported at p. May 8, in the area of Northeast Fourth Street. Top views. Makeness - Loud Patterns play album Electronic Download. Garbo - Perestroika play album Electronic Download. Alfreda Hodgson. Anthony Rolfe Johnson. Gwynne Howell. Other advantages and characteristics of the invention will become apparent in the examples and figures which follow.
The fraction adsorbed on Protein A and eluted with NaCl 0. The fractions adsorbed on a Protein G Sepharose Pharmacia derivative which has been engineered to delete the albumin binding region lane e and eluted with 0.
None of these components are present in the fraction non adsorbed on Protein A lane d , lane b contains the molecular weight markers. Figures B and C By differential elution, immunoglobulin fractions containing the 50 and 43 Kd heavy chain can be separated. Upon elution with pH 3. After column eluant absorbance has fallen to background level a second immunoglobulin component of Kd can be eluted with pH 2.
This fraction upon reduction yields a 50 Kd heavy chain and a board light chain band lane 2. After washing and elution with pH 3. M, Tris 0. After centrifugation the pellets were washed 3 times in buffer and once in buffer in which the Triton and ovalbumin had been ommitted. In all species examined the unreduced fractions Figure A contain in addition to molecules of approximately Kd also smaller major components of approximately Kd.
In the reduced sample Figure B the constituant heavy and light chains are detected. Figure A 35 S methionine labelled Trypanosome evansi antigens lysate After blocking, the membrane was extensively washed with TST buffer and incubated for 2 hours with 35 S-labelled trypanosome antigen.
After extensive washing, the membrane was dried and analysed by autoradiography. The digestion was blocked by the addition of excess iodoacetamide 4. After centrifugation of the digest in an ependorf centrifuge for 5min at rpm, the papain fragments were separated on a protein A Sepharose column into binding B and non binding NB fractions. The binding fraction was eluted from the column with 0.
Representation of a hinge region. The Kd component when reduced yields 50 Kd heavy chains and large 30 Kd light chains. The Kd fraction is totally devoid of light chains and appears to be solely composed of heavy chains which after reduction have on apparent MW of 43 Kd FIG. The fraction which does not bind to Protein G can be affinity purified and eluted from a Protein A column as a second Kd component which after reduction appears to be composed solely of 46 Kd heavy chains.
There is a possibility that these three sub classes can be further subdivided. A comparative study of old world camelids Camelus bactrianus and Camelus dromedarius and new world camelids lama pacos, lama glama, lama vicuqna showed that heavy chain immunoglobulins are found in all species examined, albeit with minor differences in apparent molecular weight and proportion.
The new world camelids differs from the old world camelids in having a larger IgG 3 molecule heavy chain immunoglobulin binding to Protein G in which the constituant heavy chains have an apparent molecular weight of 47 Kd FIG.
The abundance of the heavy chain immunoglobulins in the serum of camelids raises the question of what their role is in the immune response and in particular whether they bear antigen binding specificity and if so how extensive is the repertoire. This question could be answered by examining the immunoglobulins from Trypanosoma evansi infected camels Camelus dromedarius.
For this purpose, the corresponding fractions of IgG 1 , IgG 2 , IgG 3 were prepared from the serum of a healthy camel and from the serum of camels with a high antitrypanosome titer, measured by the Card Agglutination Test 3. This leads us to conclude that the camelid heavy chain IgG 2 and IgG 3 are bona fide antigen binding antibodies. An immunological paradigm states that an extensive antibody repertoire is generated by the combination of the light and heavy chain variable V region repertoires 6.
The heavy chain immunoglobulins of the camel seem to contradict this paradigm. Immunoglobulins are characterized by a complex I. To determine whether the two heavy chains constituting the IgG 2 and IgG 3 are identical or not, the isoelectric focussing I.
As this alkylating agent does not introduce additional charges in the molecule, the monomers resulting from the reduction and alkylation of a heavy chain homodimer will have practically the same isolectric point as the dimer, whereas if they are derived from a heavy chain heterodimer, the monomers will in most cases differ sufficiently in isoelectric point to generate a different pattern in I.
Upon reduction, and alkylation by iodoacetamide the observed pattern is not modified for the Camelus dromedarius IgG 2 and IgG 3 indicating that these molecules are each composed of two identical heavy chains which migrate to the same position as the unreduced molecule they originated from.
In contrast, the I. These findings indicate that the heavy chains alone can generate an extensive repertoire and question the contribution of the light chain to the useful antibody repertoire. If this necessity be negated, what other role does the light chain play. Normally, isolated heavy chain from mammalian immunoglobulins tend to aggregate considerably but are only solubilized by light chains 8, 9 which bind to the C H 1 domain of the heavy chain. In humans and in mice a number of spontaneous or induced myelomas produce a pathological immunoglobulin solely composed of heavy chains heavy chain disease.
The reason why full lenght heavy chains do not give rise to secreted heavy chain in such pathological immunoglobulins seems to stem from the fact that the synthesis of Ig involves a chaperoning protein, the immunoglobulin heavy chain binding protein or BIP 11 , which normally is replaced by the light chain It is possible that the primordial role of the light chain in the four-chain model immunoglobulins is that of a committed heavy chain chaperon and that the emergence of light chain repertoires has just been an evolutionary bonus.
This would suggest that deletions have occurred in the C H 1 domain. The strategy followed for investigating the heavy chain immunoglobulin primary structure is a combination of protein and cDNA sequencing; the protein sequencing is necessary to identify sequence streches characteristic of each immunoglobulin. The N-terminal of the immunoglobulin being derived from the heavy chain variable region repertoire only yields information on the V HH subgroups variable region of the heavy chain and cannot be used for class or subclass identification.
This means that sequence data had to be obtained from internal enzymatic or chemical cleavage sites. A combination of papain digestion and Protein A affinity chromatography allowed the separation of various fragments yielding information on the general structure of IgG3. The IgG3 of the camel Camelus dromedarius purified by affinity chromatography on Protein A Sepharose were partially digested with papain and the digest was separated on Protein A Sepharose into binding and non binding fractions.
The bound fraction contained two components, one of 28 Kd and one of The precipitated protein was collected in a pellet by centrifuging rpm, 5 min and was used for protein sequencing.
N-terminal sequencing was performed using the automated Edman chemistry of an Applied Biosystem A pulsed liquid protein sequencer. All chemical and reagents were purchased from Applied Biosystems. Analysis of the chromatographic data was performed using Applied Biosystems software version 1. In every case the computer aided sequence analysis was cofirmed by direct inspection of the chromatograms from the PTH analyser. Samples of dissolved protein equivalent to pmol 28 Kd fragment or pmol 14Kd fragment were applied to TFA-treated glass fibre discs.
The glass fibre discs were coated with BioBrene 3 mg and precycled once before use. The N-terminal sequence of the Upon reduction, a single diffuse band of 17 Kd is found indicating that the 34 Kd is a disulfide bonded dimer of the 17 Kd component. It has been shown that the cells from camel spleen imprint cells reacted with rabbit and anti camel immunoglobulin sera and that the spleen was hence a site of synthesis of at least one immunoglobulin class.
The conditions for the isolation of RNA were the following: total RNA was isolated from the dromedary spleen by the guanidium isothiocyanate method The availability of this sequence data allowed the construction of an exact 3' primer and the cloning of the region between the N-terminal end of the V HH and the C H 2 domain. After amplification the PCR products were checked on a 1. A final phenol-chloroform extraction preceded the ligation into pUC 18 series 56 clones or into pBluescript R series 72 clones.
All the clones obtained were smaller that the base pairs to be expected if they possessed a complet V HH and C H 1 region. Partial sequence data corresponding to the N-terminal of the V HH region reveals that out of 20 clones, 3 were identical and possibly not independent. Clones corresponding to two different sets of C H 2 protein sequences were obtained. The C H 1 domain is entirely lacking. These results have allowed us to define a model for the IgG3 molecule based on sequence and papain cleavage FIG.
Papain can cleave the molecule on each side of the hinge disulfides and also between C H 2 and C H 3. Under non reducing conditions the V HH domains of IgG3 can be isolated as disulfide linked dimer or as monomer depending on the site of papain cleavage. Such second set of clones could represent the IgG2 subclass. Several V HH regions cDNA's have now been totally or partially sequenced with the exception of a short stretch at the N-terminal end which is primer derived.
Upon translation the majority shows the characteristic heavy chain Ser 21 CyS 22 and Tyr 90 Tyr 91 Cys 92 sequences, of the intra V HH region disulfide bridge linking residues 22 and All these clones have a sequence corresponding to the framework 4 FR4 residues of the variable region immediately preceding the postulated hinge sequence Table 3. This sequence is generated by the J minigenes and is in the majority of cases similar to the sequence encoded by the human and murine J minigenes.
The sequence length between region Cys 92 and the C-terminal end of the V HH regions is variable and, in the sequences determined, range from 25 to 37 amino-acids as one might expect from the rearrangements of J and D minigenes varying in length. Several important questions are raised by the sole existence of these heavy chain immunoglobulins in a non pathological situation. First of all, are they bonafide antibodies?
The heavy chain immunoglobulins obtained from trypanosome infected camels react with a large number of parasite antigens as shown in part I of these examples. This implies that the camelid immune system generates an extensive number of binding sites composed of single V HH domains. The second question is "how are they secreted?
The secretion of immunoglobulin heavy chains composing four-chain model immunoglobulins does not occur under normal conditions. A chaperoning protein, the heavy chain binding protein, or BIP protein, prevents heavy chains from being secreted. It is only when the light chain dispplaces the BIP protein in the endoplasmatic reticulum that secretion can occur The heavy chain dimer found in the serum of human or mice with the so-called "heavy chain disease" lack the C H 1 domains thought to harbour the BIP site In the absence of thi domain the BIP protein can no longer bind and prevent the transport of the heavy chains.
The deletion of C H 1 domain in the two types of heavy chain antibodies devoid of light chains, already sequenced has, as a result, a modification of this maximum span FIG. This could be a severe limitation for agglutinating or cross linking. In the IgG2 this is compensated by the extremely long stretch of hinge, composed of a fold repeat of the sequence Pro-X where X is Gln, Lys or Glu and located N-terminal to the hinge disulfide bridges. In contrast, in the human IgG3, the very long hinge which also apparently arose as the result of sequence duplication does not contribute to increase the distance spanning the two binding sites as this hinge is inter-spersed with disulfide bridges.
The single V HH domain could also probably allow considerably rotational freedom of the binding site versus the Fc domain. Unlike myeloma heavy chains which result probably from C H 1 deletion in a single antibody producing cell, or heavy chain antibodies produced by expression cloning 15 ; the camelid heavy chain antibodies devoid of light chains have emerged in a normal immunological environment and it is expected that they will have undergone the selective refinement in specificity and affinity accompanying B cell maturation.
However, the 3' end of the gene was not in phase with the identification "tag" and the stop codon of the vector. To achieve this, the construct was cut with Spe and the 4 base overhangs were filled in, using the Klenow fragment after which the vector was religated. The expression vector plasmid ipBS immunopBS Stratacyte contains a pel B leader sequence which is used for immunoglobulin chain expression in E.
In addition, it contains a sequence for a c-terminal decapeptide tag. The suspension was kept on ice for 2 hours. The supernatant periplasmic fraction was dialysed against Tris-HCl pH 8. As can be seen, the major impurity is eliminated by ionexchange chromatography and the bulk of the remaining impurities are eliminated by gel filtration.
Ward, E. Nature , Edelman G. Roitt I. New-York, p. Seligmann M. Henderschot L. Hamers-Casterman, C. Se a vel. L cual. Pero qua me sequel osi. Y on memento tan oportuno. Ante la responsobilldad profesional del FAum y ante Ia responsabilidad oficial'de las autoridades no puede quedar ol asunto sin una dilucidacio plena.
Ante [a responoobilidad cfval df olos, con lo informes, lo dictimenes y fee aclaraciones del F6rum casarin paro siempre lUs intromisiones meZquinoas de lo politiquilla quo seialibamos an editorial reciente y quo lo realidad doJ hecho-hiace mis impugnable do-lo quo un pudor patri6tlco nos permitinera consignaor oloeces. Crimenes rojos UNA vex mi, me pone at desnudo an. Otro roll is verdad do wiare que abstie- doscientas futeran arrojadoz Par at ne a] communism y empapa su ban- oinl vertical douna mina, Y mi si-a Sign reports faciltatin per do cuairoaleat fueonn.
Y mis de dos- todo y cada unode loo crimene cienlos cincuenta mil naturalen han on msa perpetrados per los rujos corrido lguan ausrte. Ante' taaoia salvajodo pudiero tdos, sltan A Ia vista qu el munpensar o in I. Pero Is minucions fidelidad land log mis olementales prinri. Derecho, quier arrastrr forme I general Hanley, no de. A despecho de Ea inatl. Son-qrlmsoaes roles a seems: Anilo- crmente las excelaitude del restmal; 'a Ioo comstidos poe 1 comu- men ovitico, presentindolo corn glmo tod lo ;largo y to ancho lanmbre y meta de oas aspiraclo.
La Irre. Segdo al reports, entire el 10 y futable realidod de los hechos de. El 10 is diciombre del in do Is vilencla y I -sternatita. In iistotio, a travix da doo on las inmediloa nes de Is lo Agnlos. Dee m prisione- rigimen ms sanorient qut ai co. El tinoo recubso que quedo, do Is or usladamoente pueblos o In ontenda, con el propsito de debiltrI las fuers de Ins grades potencies, come, ha sucedido en Core, tamblie ha Ido perdendoof-. I a rsa efectista del.
Y come conoetueneia, repercuten la palobras de une voz norteamericanen Is Asamblea. Pr leycnal0,,el hnobre sle. Icednoeatl hohbroasaltar oI desrado, y meter to discordla donde reIna In paz. El objeto ed lugrar el oayer rendlmientocon cin00orereo. Per pee. Con una ingenuldd quo fura deliclosa, i noentraoitra tanta peIgromaddd. Al a01Continents on pidmicas y amenazas, entre too pueblos hermanos; y en dimes y dire. Una Amrica desunids es la quo perslgueI s Unlie sovitic.
Para sue planes; y nostros, tan onfaoile p cnedidne fem co,I1 niablizamosel onlic. Qod mA3 quislera Rusa que una Am, c latno. Pero, per tfrtuna, Ia tropia economic. Aceptemos quo Estado Unidoo 1It ejerco; pero no olvidemos, a mismontiempo, quea Ino ame-n0 dos Imperiallsmos: uno es el del dilar; pro el otos ei do lo e uemr y el extrninie, que es el de Rust.
C6mo andarian I. Entonces hubinra querido ver-yo- mis-de-un-ap6stol-sociala politico, de os quenom astamos, y quutilllan come uno de sus rucon. Imprelonntes sum"convo. Con el- imperiiamo del dilar, comemos, tonemos lobertd, mTdramo a nuetro in oinJo; con el. Y hs eros del Terarr on oranci y del hillerlann 0nAl. Hermosoen conceptos. Sentad oo cipto. Su santidad explica Is presencia do Diem ia ddivi 1n del tono a In expansion del UniverAnte Is Corptrcidn lustre, anpalabra fue Consendo el argument s6ido, irrebatible.
Co Sanidadconecreto. Congreso de minoristas L 09 detallst. La de este alio ho logrado wrogregar mis de ochenta delegacione Como siempre ocurre n esIa cle de eventos, do prograras se presentaron a Il consideracin del de legado: 01 de ogaxajos, que como visitante distinguid se le ofrece, y el que- ontiene 01 tmari de trabajo a entudiar, discutir y aprobr. I necesidad diramno, do crear n Banco espscialment dedicado a operactones en ram s ten importante d ioeelvnhilelto mmorccInh Todavnlo spec coteuab.
Is Centneri o renmedo pyaprnbando mociones dielsig Oe la rolCU fueron aceptadas el dia ante rior. Per tal se est in- yor irresponsablidad 7cegern.
Peso ot ar- abio articunde do orr. Digo esto, porque el de galeria. Repblica se destu- tregrselas; y ;puede l estado am0to, all rIla. Hace falls mucho va- me rer presa detal asgnitud. Rail de Cir- com o el escrlbno del ent, qua - doeas0a no vordode rr ode do tre lls trmblin s ire del CatInRepubbe. Roil de CArdenas sirve lnteaumentue n prapeCone - a a Repinbic en to permanente. Aquelloe' que per dema- mor aloleromnlima. Yer no 0uins. L" ct- e00 ae 11dpeIoradlOn dola be- pables son estos dibilen dudada- nras seobre Iop hcendados pre er nos que "pr no caer peados mo- do sliUrs con rI sy, a ells t-.
El doa t Repbblrca, cundo no crl bo- dliue muer un colony, n nMRdirid I di undo al o r I r ooe dn ado e t vu a: seor e". Y ye ar ors ElTiempo triturado "Nostradamus red"o ondiane bay e rear, recu.
F i oW l-mon sos aoigoo y no po- ano p ol omndb t 1 ActI- callooldad" 0n l monos lua mues. E c d n aberAprodo. Roberts, slo a. Ya En pleno uelo ac bai no o rc breri d qil telCa vinl so so. LO que nootoigloXVldo sda". Le- Nostradom soe nderab o0' yo suingo.
Con el cambio do normand, viden- Oomooa o toro6gleaasig d soqud. Ilnuestra propa hora; IA radio nos Fue laI ls qu hon estudido el doctor Rhine de so dbo convertir do arrendatarlo El Tiempo hsido, dislocado, des- do las canpana del relod i la vaticin6 a De.
Antesun ahoraeraUna co. El sustantivo timpo novia'de venidd de Nueva York. Para Mr. Y l tele-yunique prios ens psr lode 01 Uninoro. Como charla a su ober con No. Y bonito show va nom; coraues contiahor nou. El mo emosoa slo- rintro. El del rctraemore. Lo o mal y omirets tanto el favorlca 11or adpoemadsmrent r0 Madrid, de n deBudapest, de pued Interpretr air border n deduce de varies estrofas nostr- irma.
Hle anesteomureta,]abad dcase. EraolrelbdestIa mgre. S el esol. El one Is o ra om- codas en Franciaohace i-o. Otra emrOf lento de DonWnicold Riverhcere.
Cuando derpudo que1 I: "Las estrofas profticas acontecimientos n Espafa: -L prosencia de an bmrte de demagotIutax qu0 partio un. Ro- de. Me hoe mle en ora pAgina sde su ledoo Actuacudodoo: RN. En cart rastrn Ierresistiblemente a lo Monteyp.
La idea del Tempo h sido tr. Nostradamus afr. En cualquier lafenin, -Ls sr6. Puede lmber Jos y pr el porvenir de Cubal pe clerta imsortnci. I DivineElencia me h reve- duda. Roberto, quo an. Suyo affmo. Cnraz6nose vuelven so? Para qu yo no imga duda Bail do Cirdona. Roberto-obre -o -evnt- crey6 todo perdido porque-"ets aOd. La o toamino rrcirueleennion c ioa. Dep-r--obra de Amima. Ryen y td odoshacia 2Mno2iChI. EscndinsI", toO.
El sig- En vista sin dud. Roberts me lee troou de une Ahora segumos hablnde ivi e ami liasdame- croaesrelo. Roberts: "In- carta do Nostradamus al Rey Enri- de ploa lobien me]; ai ,acdticouerto.
UnA epoca "tumultuos-r e00 rrpueode ls. Po otra etrota dice: 'era de renovac16n-"' p cuminar n cone-trmddenerg o las tW. Ins do. Oue l uustra, pue n. Rorber ton. N11,5 ino em un hobre an Ta'eas ee Alana d las Estado. Unidos de cia y renultar mis-un cornIeom o cnc homlr neinounadeo- or' e doiamate,, Por unos e- las Anmrtias y leo Etado Tildo que un Pincipe El fracasio do blirasdm ste. Lig do los HNodnes snu estol -Ildod.
Caguario s n nu SoIP el ro igo. Lso ao oenegui dao per ntmean ahi Sevn srdamum oiu exeets ci sa etra 0n NostradamUS: hia convrudo, Mr. E on va 0,per o y y ntro depoco 36 seriC op nnus- eon as y guerras, pern desputs rinra oeranPrturbadores; los maestroee Interlocuior spiritual puc pretend evIdirl aumentando f. Die cuando ahogue ao lonhumanidd en CIidr"tiIceso b. Es -br aye tampoco adIlremos ala Y ycalm en su segun isedici'n; fls e0n el cielo". Eote sviso del do oi old. Caind tormilrpo 'lioempapado unaha clen vecsii itortt a rs Mud' tpuhb"i en0 td de soc.
El antigiobreln-s 9y on. Saun Mr Roberts. En alina dpoca entree el editor, duode no se habian pu- to nsalvr - - Crn'ne. Morales Y d la noche, brindaran un cocktaillu hlllnlon esposa Marit Tren Co- n dencla della l elnotoo mey n ptolrd,.
Aveeldn, n Miramar, e copodedd, u rrtba e di eeu'. B lnon dn Platn. Qecnhc n coan 'Qu. Alonso Forcade y Jorrin '1 No Iapresuraen-oroce. La Habae. Miguel La. Model Trianon, I Ercus 24 piezs Ifide pao teles, resteaeanfe obwindad da shjena pari rejgel rfr o 0a W. Morceino I 5-o gn od51, Apd 26plnoun Daffodil gen lea.
El gran fashion show de maiinna Estamos en vlisperit de un sconte- dinaria belle. Ademnd "Lea Cha-len" menn aran iry ensu targo y magnftc. Tedo p. In El Coeit. CaroluOlavacrimde Mendo- lago mypliod emn lgo eo, ya 7. Litl n que suo denon. Alina John. Ia do anhen, rota de 'er-lo pode. Y a toe diez menos cuarto seini- m. I, d tesingular elegancia, de extraor. Complenoautudar en primer. Nena Pella. Ia elegante espoaendelas d.
I etegaptenedoca de del senor Luis JunadeIla. CadoI' dl eddo Flera G Seri a is cineco deIla tarde. Dala Medina Madrua. Itimado compadero Willy del Pinon. La encantadraBebita Snchee. Hab nE. Pleran, broches. M y A-7 a. Con In ilueta del taln ycostura en ne ro y carmelita. Custro prerionas tonalidade, en todasI tn. Adldione Plant. Magnificop par. Puede obtenerls de cordon fino o gre. TelasnI Polu aboe -? Madelas do lona on colored firm.
Hilos y Srderia: Plants bsjta Juego do 8 vasos para n"highballs" 3. Vasode fino Cnrs tal, con 6' de alto, base doble, y artistica decoreaida deun caballo negro con detaliesen blanco. SO De O Dobecapacdad I tchos de alambre.
Capacidad para 44 platoy p ebieCton. Iido do Jersey S3. Se ofrecen' en azul, main o verde con finnaWneramnoeelatmaa do. Taltan:pequeda, mediana ygrcader. Sport de ora: nSeuna p" rn Pigina 5'. A po a virtud de u na denunia formuladia e "Presidencia, dijo el Dr. Illr e oea na e apovn e LV motnecml eiifai. Mod 16, Redlc eerre i e- d-. So n istr do Educ g- ac i n nirn 1u. Fbi aes e feo to'trio am'e ae u b6a otr"uo e e lt d'rM. EwnnaPiteg ta t rsame acaeca s onadesa d aue asfr o ahind d. Sacof, Il.
Co- d. Aa 5 15 Pr rtrpobea eraia C ota. AU ddo anuncianP1 1. Jao-el'cniaualso Im eI. S -1iv consider debi 25b miemb E l eo Inc de -Vla, eei R. Y ue steacerd sftrnr-Prr dhde Publ CuI.
I- un r- -is' e e11 cotendedoM. Clan j 4 aw am i niawrasD r Ltn c,. Smt E. Ior San Cr: jebl do petoa nsmla muI cp,. La il n. It ,h en enr y. A uct. Qtnet 1, ay ATida- 1Is i rnt afgr nplrqe 12lo 2 dq. Ed cuel. Yi s an rra. I0 1' den Abll. In7 in2 dinrdn u in Asone -doc to 1.
We -,. Aamle Mnia. M 6" dos pro ble aede dd. G line lar. Si no lo prob6, Iprudbelo 'ehorol No querr6 otro. El Dr.A a d que estos puebl)3 nuelitrols debiRn 19 60 00 anillo al dedo" critics So le ha exteriorizado con. conlo el desti- ser respetaticis en el empefic, de Ano dc minicai 5 go 8 10 cual sueli dear- muestras cilidas de respeto Y often- no-, c a 0 Is con- 1 -1 ki neinniticar Ins conditions tic, unR.